ARTIFICIAL MAINTENANCE MEDIA FOR CELL AND ORGAN CULTIVATION

Artificial Maintenance Media for Cell and Organ Cultivation

Experiments have been described in which organs cultivated in the Lindbergh apparatus have been kept alive in artificial media for periods ranging from 3 days to 3 weeks, with life continuing in portions of some glands for from 35 to 62 days. The media used varied in composition from very simple and inexpensive ones in which some serum was used to a rather complex medium containing only that ne...

Artificial M a I N T E N a N C E Media for Cell and Organ Cultivation I. the Cultivation of Fibroblasts in Artificial and Serumless Media* by Lillian

The importance of developing artificial media that can be used in the place of serum for maintaining the life of tissues and organs outside the body hardly needs to be emphasized. Many of the studies for which the organ culture technique 1 was developed, as well as others that can be carried on by the simpler methods of tissue culture, depend for their success on the creation of suitable artifi...

Artificial cells for cell and organ replacements.

The artificial cell is a Canadian invention (Chang, Science, 1964). This principle is being actively investigated for use in cell and organ replacements. The earliest routine clinical use of artificial cells is in the form of coated activated charcoal for hemoperfusion for use in the removal of drugs, and toxins and waste in uremia and liver failure. Encapsulated cells are being studied for the...

Medium development beyond production media: Chemically defined media for transfection and single cell cultivation

Background State-of-the-art medium and feed development has proven its potential for increasing bioprocess efficiency many times. By the application of such advanced chemically defined and animal component-free formulations, in combination with recent cell lines, the yield of mammalian cell cultures was pushed to the binary gram per liter level. In this work, the current progress in designing s...

Organ Donation Awareness: Rethinking Media Campaigns